Preparation process of β-Nicotinamide Mononucleotide

The preparation process of β-Nicotinamide Mononucleotide is summarized as follows: first,β-Nicotinamide Mononucleotide nicotinamide ribose salt is phosphorylated under alkaline conditions to obtain nicotinamide mononucleotide salt,β-Nicotinamide Mononucleotide and then acidified under acidic conditions to obtain the target initial product, and then After the purification step,β-Nicotinamide Mononucleotide the pure product of the target product is obtained. The reaction formula is as follows: The preparation method of β-nicotinamide mononucleotide specifically includes the following steps: phosphorylation reaction,β-Nicotinamide Mononucleotide in an alkaline aqueous solution of pH 8-10, the temperature of the reaction system is Under the condition of 25-50°C,β-Nicotinamide Mononucleotide the nicotinamide ribose salt with the formula structure and the metaphosphate are subjected to a phosphorylation reaction for 2-5 hours to obtain an intermediate product with the structure of the formula iii.

Acidification reaction: add acid to the reaction solution,β-Nicotinamide Mononucleotide adjust the pH value to 3 to 5, and lower the temperature of the reaction system,β-Nicotinamide Mononucleotide acidify the intermediate product with formula structure to form β-nicotinamide mononucleotide with formula i structure; product purification, The reaction solution after step b is filtered through protein membrane, concentrated by high-pressure desalting membrane, separated and purified by anion exchange resin,β-Nicotinamide Mononucleotide and concentrated and crystallized to obtain pure β-nicotinamide mononucleotide.

Preferably,β-Nicotinamide Mononucleotide in step a, m in the formula is sodium or potassium, metaphosphate is sodium metaphosphate or potassium metaphosphate,β-Nicotinamide Mononucleotide and the content of sodium trimetaphosphate in metaphosphate is calculated as p2o5, and the content is greater than 68%,β-Nicotinamide Mononucleotide 1 The ph of% aqueous solution is 6.2. The alkaline aqueous solution used is any one of sodium hydroxide, potassium hydroxide,β-Nicotinamide Mononucleotide lithium hydroxide, calcium hydroxide and magnesium hydroxide aqueous solution. The pH value of the reaction system is 9,β-Nicotinamide Mononucleotide the reaction temperature is preferably 30°C, and the reaction time is 4 hours. Through online reaction monitoring,β-Nicotinamide Mononucleotide the raw materials are consumed after 4 hours of reaction.

Tag: Β-Nicotinamide Mononucleotide

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