Mar 13, 2021 / Author: China Glutathione suppliers & NMN manufacturers
The preparation process of β-Nicotinamide Mononucleotide is summarized as follows: first,β-Nicotinamide Mononucleotide nicotinamide ribose salt is phosphorylated under alkaline conditions to obtain nicotinamide mononucleotide salt,β-Nicotinamide Mononucleotide and then acidified under acidic conditions to obtain the target initial product, and then After the purification step,β-Nicotinamide Mononucleotide the pure product of the target product is obtained. The reaction formula is as follows: The preparation method of β-nicotinamide mononucleotide specifically includes the following steps: phosphorylation reaction,β-Nicotinamide Mononucleotide in an alkaline aqueous solution of pH 8-10, the temperature of the reaction system is Under the condition of 25-50°C,β-Nicotinamide Mononucleotide the nicotinamide ribose salt with the formula structure and the metaphosphate are subjected to a phosphorylation reaction for 2-5 hours to obtain an intermediate product with the structure of the formula iii.
Acidification reaction: add acid to the reaction solution,β-Nicotinamide Mononucleotide adjust the pH value to 3 to 5, and lower the temperature of the reaction system,β-Nicotinamide Mononucleotide acidify the intermediate product with formula structure to form β-nicotinamide mononucleotide with formula i structure; product purification, The reaction solution after step b is filtered through protein membrane, concentrated by high-pressure desalting membrane, separated and purified by anion exchange resin,β-Nicotinamide Mononucleotide and concentrated and crystallized to obtain pure β-nicotinamide mononucleotide.
Preferably,β-Nicotinamide Mononucleotide in step a, m in the formula is sodium or potassium, metaphosphate is sodium metaphosphate or potassium metaphosphate,β-Nicotinamide Mononucleotide and the content of sodium trimetaphosphate in metaphosphate is calculated as p2o5, and the content is greater than 68%,β-Nicotinamide Mononucleotide 1 The ph of% aqueous solution is 6.2. The alkaline aqueous solution used is any one of sodium hydroxide, potassium hydroxide,β-Nicotinamide Mononucleotide lithium hydroxide, calcium hydroxide and magnesium hydroxide aqueous solution. The pH value of the reaction system is 9,β-Nicotinamide Mononucleotide the reaction temperature is preferably 30°C, and the reaction time is 4 hours. Through online reaction monitoring,β-Nicotinamide Mononucleotide the raw materials are consumed after 4 hours of reaction.
Supplier Introduction: China glutathione supplier and NMN manufacturer GSHworld, the company mainly develops biotechnology and industrialization. As a global pioneer in enzymatic catalytic ATP regeneration technology, our company advocates green production and is committed to providing customers with better and more environmentally friendly products and services. Glutathione Manufacturer,NMN Factory,Citicoline Sodium supplier,China NMN manufacturers